Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR (Ab-2481).| Product Name | mTOR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FRAP, FRAP1, FRAP2, RAFT1, RAPT1, ERAB, HADH2, MRPP2, SCHAD, XH98G2, FRAP, Rapamycin target protein |
| Product description | Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.1) Sabers, C. J. et al. (1995) J. Biol. Chem. 270, 815-822. Brown, E. J. et al. (1994) Nature 369, 756-758. Dennis, P. B. et al. (2001) Science 294, 1102-1105. Sabatini, D. M. et al. (1994) Cell 78, 35-43. |
| Immunogen | mTOR (Ab-2481) antibody was raised against a peptide sequence around aa.2479~2483 (I-H-S-F-I) derived from Human mTOR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTOR |
|---|---|
| Alternative Names | FRAP FRAP1 FRAP2 RAFT1 RAPT1 ERAB HADH2 MRPP2 SCHAD XH98G2 FRAP Rapamycin target protein |
| Molecular Weight(MW) | 289 kDa |
| Tissue Specificity | This antibody detects endogenous level of total mTOR protein. |
| Entrez Gene | 2475 |
|---|---|
| Protein Accession | NP_004949.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR (Ab-2481).| Application Notes | Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
