Application
Western blot analysis of extracts from NIH-3T3 cells treated with Insulin using Phospho-mTOR (Thr2446) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | mTOR (phospho Thr2446) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.1) Holz MK, Blenis J (2005) J Biol Chem 280, 26089-93 |
| Immunogen | mTOR (phospho Thr2446) antibody was raised against a peptide sequence around phosphorylation site of threonine2446 (T-R-T (p)-D-S) derived from Human mTOR . |
| Modification | p-Thr2446 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTOR |
|---|---|
| Alternative Names | FRAP FRAP1 FRAP2 RAFT1 Rapamycin target protein |
| Molecular Weight(MW) | 220 kDa |
| Tissue Specificity | The antibody detects endogenous level of mTOR only when phosphorylated at threonine 2446. |
| Entrez Gene | 2475 |
|---|---|
| Protein Accession | NP_004949.1 |
Application
Western blot analysis of extracts from NIH-3T3 cells treated with Insulin using Phospho-mTOR (Thr2446) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
