Application
Confocal immunofluorescent analysis of MUSK Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti-MUSK Pab in mouse kidney tissue lysates (35ug/lane)| Product Name | MUSK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MuSK activates signaling cascades responsible for many aspects of synapse formation, including the organization of the postsynaptic membrane, synapse-specific transcription, and presynaptic differentiation. MuSK also mediates agrin-induced aggregation of acetylcholine receptors at the mature vertebrate neuromuscular junction and also during synapse formation. It has been shown that a majority of AChR Ab-seronegative myasthenia gravis patients possess serum autoantibodies against MuSK.1) Blume-Jensen P, et al. Nature 2001. 411: 355. |
| Immunogen | This MUSK antibody is generated from rabbits immunized with human MUSK recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MUSK |
|---|---|
| Alternative Names | Muscle skeletal receptor tyrosine-protein kinase Muscle-specific tyrosine-protein kinase receptor MuSK Muscle-specific kinase receptor MUSK |
| Molecular Weight(MW) | 97 kDa |
Application
Confocal immunofluorescent analysis of MUSK Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti-MUSK Pab in mouse kidney tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
