Myb-Related Protein B (MYBL2), a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.1) Tashiro, S., et al., (2004) J.Biol.Chem.279(17), 17715-17722.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MYBL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
MYBL2 antibody can be used for detection of MYBL2 by ELISA at 1:62500. MYBL2 antibody can be used for detection of MYBL2 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Spermatophore.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
MYBL2 antibody can be used for detection of MYBL2 by ELISA at 1:62500. MYBL2 antibody can be used for detection of MYBL2 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MYBL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.