Application
Western Blot at 1:1000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MYCN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYCN is a member of the MYC family and a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of its gene is associated with a variety of tumors, most notably neuroblastomas.1) Combaret,V., Pediatr Blood Cancer 53 (3), 329-331 (2009) |
| Immunogen | This MYCN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-351 amino acids from the C-terminal region of human MYCN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MYCN |
|---|---|
| Alternative Names | N-myc proto-oncogene protein Class E basic helix-loop-helix protein 37 bHLHe37 MYCN BHLHE37 NMYC |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:1000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
