Application
Immunohistochemical staining of human heart tissue using MyD88 antibody at 2 μg/mL.Application
Immunofluorescence of MYD88 in Human Testis cells with MYD88 antibody at 20 μg/mL.Application
Western blot analysis of MyD88 in (A) HeLa and (B) Jurkat cell lysate with MyD88 antibody at 1 μg/ml.| Product Name | MYD88 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYD88 Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.1) Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5 |
| Immunogen | MD88 antibody was raised against a 16 amino acid peptide near the center of human MD88. The immunogen is located within amino acids 220 - 270 of MYD88. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MYD88 antibody can be used for detection of MyD88 by Western blot 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYD88 |
|---|---|
| Alternative Names | MYD88 Antibody: Myeloid differentiation primary response 88 MYD88D |
| Molecular Weight(MW) | Predicted: 35 kDa Observed: 36 kDa |
| Sequence Similarities | 4) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunohistochemical staining of human heart tissue using MyD88 antibody at 2 μg/mL.
Application
Immunofluorescence of MYD88 in Human Testis cells with MYD88 antibody at 20 μg/mL.
Application
Western blot analysis of MyD88 in (A) HeLa and (B) Jurkat cell lysate with MyD88 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MYD88 antibody can be used for detection of MyD88 by Western blot 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MYD88 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MYD88 Antibody is supplied in PBS containing 0.02% sodium azide. |
