Application
Immunofluorescence of MyD88 in Jurkat cells with MyD88 antibodyApplication
Western blot analysis of MyD88 in Jurkat whole cell lysate with MyD88 antibody at 1 μg/mL.| Product Name | MYD88 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYD88 Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.1) Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5 |
| Immunogen | MD88 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human MD88. The immunogen is located within the last 50 amino acids of MYD88. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| WB:1:500~1:2000 IF:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYD88 |
|---|---|
| Alternative Names | MYD88 Antibody: MYD88D |
| Molecular Weight(MW) | Aprox: 35 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunofluorescence of MyD88 in Jurkat cells with MyD88 antibody
Application
Western blot analysis of MyD88 in Jurkat whole cell lysate with MyD88 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | MYD88 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MYD88 Antibody is supplied in PBS containing 0.02% sodium azide. |
