Application
Immunohistochemistry staining of MYD88 in skin tissue using MYD88 Antibody.| Product Name | MYD88 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MyD88, a protein involved in interleukin-1 (IL-1) mediated signalling was originally isolated as myeloid differentiation primary response gene (3,4). MyD88 possesses a N-terminal death domain similar to cytoplasmic segments of TNF receptor 1, Fas, and C-terminal region related to IL-1 and Toll receptors. Overexpression of MyD88 induces activation the c-Jun N-terminal kinase and NF-κB through its death domain (1,2). The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-κB activation induced by IL-1, but not by NF-κB. The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues. |
| Immunogen | MYD88 antibody was raised against amino acids 233 - 248 of MYD88 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| MYD88 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | MYD88 |
|---|---|
| Alternative Names | MYD88 MYD88D |
| Tissue Specificity | Because of high sequence homology, this is expected to detect multiple isoforms of MyD88 protein. |
Application
Immunohistochemistry staining of MYD88 in skin tissue using MYD88 Antibody.| Application Notes | MYD88 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MYD88 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.02% sodium azide. |
