Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).| Product Name | Myeloperoxidase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq].1) Banerjee, M., et al. Toxicol. Appl. Pharmacol. 249(1):47-54(2010) |
| Immunogen | This Myeloperoxidase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human Myeloperoxidase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPO |
|---|---|
| Alternative Names | Myeloperoxidase MPO Myeloperoxidase 89 kDa myeloperoxidase 84 kDa myeloperoxidase Myeloperoxidase light chain Myeloperoxidase heavy chain MPO |
| Molecular Weight(MW) | 84 kDa |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
