Application
Myeloperoxidase Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Myeloperoxidase Antibody for immunohistochemistry.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).| Product Name | Myeloperoxidase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq].1) Banerjee, M., et al. Toxicol. Appl. Pharmacol. 249(1):47-54(2010) |
| Immunogen | This Myeloperoxidase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-448 amino acids from the Central region of human Myeloperoxidase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPO |
|---|---|
| Alternative Names | Myeloperoxidase MPO Myeloperoxidase 89 kDa myeloperoxidase 84 kDa myeloperoxidase Myeloperoxidase light chain Myeloperoxidase heavy chain MPO |
| Molecular Weight(MW) | 84 kDa |
Application
Myeloperoxidase Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Myeloperoxidase Antibody for immunohistochemistry.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
