MYF5 is a member of the myogenic basic helix-loop-helix family of transcription factors, which can activate the muscle differentiation program.1) Kosek, D.J., (2006) J. Appl. Physiol. 101 (2), 531-544.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MYF5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
MYF5 antibody can be used for detection of MYF5 by ELISA at 1:312500. MYF5 antibody can be used for detection of MYF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse skeletal at: 1:1000 (Lanes: 1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate).
Positive Control
1) 721_B Cell Lysate
Application Notes
MYF5 antibody can be used for detection of MYF5 by ELISA at 1:312500. MYF5 antibody can be used for detection of MYF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MYF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.