MYL6 contains 3 EF-hand domains. It is the regulatory light chain of myosin. MYL6 does not bind calcium. Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene.1) Fu, Z.Y., (2006) Acta Biochim. Biophys. Sin. (Shanghai) 38 (9), 625-632.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MYL6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
MYL6 antibody can be used for detection of MYL6 by ELISA at 1:1562500. MYL6 antibody can be used for detection of MYL6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
MYL6 antibody can be used for detection of MYL6 by ELISA at 1:1562500. MYL6 antibody can be used for detection of MYL6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MYL6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.