Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of APP-BP1 using rabbit polyclonal APP-BP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2).Application
Western blot analysis of anti-APPBP1 Antibody in mouse brain tissue lysates (35ug/lane).| Product Name | NAE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | APPBP1 binds to the beta-amyloid precursor protein, a cell surface protein with signal-transducing properties thought to play a role in the pathogenesis of Alzheimer's disease. In addition, this protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. APPB1 is required for cell cycle progression through the S/M checkpoint.1) Chen,Y., J. Cell Biol. 163 (1), 27-33 (2003) |
| Immunogen | This NAE1 (APPBP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-459 amino acids from the C-terminal region of human NAE1 (APPBP1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NAE1 |
|---|---|
| Alternative Names | NEDD8-activating enzyme E1 regulatory subunit Amyloid beta precursor protein-binding protein 1 59 kDa APP-BP1 Amyloid protein-binding protein 1 Proto-oncogene protein 1 NAE1 APPBP1 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of APP-BP1 using rabbit polyclonal APP-BP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2).
Application
Western blot analysis of anti-APPBP1 Antibody in mouse brain tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
