Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | NCAPH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCAPH, BRRN1, CAP-H, HCAP-H |
| Product description | NCAPH is a member of the barr family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization.1) Beausoleil, S.A., (2006) Nat. Biotechnol. 24 (10), 1285-1292. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCAPH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NCAPH antibody can be used for detection of NCAPH by ELISA at 1:62500. NCAPH antibody can be used for detection of NCAPH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCAPH |
|---|---|
| Alternative Names | NCAPH BRRN1 CAP-H HCAP-H |
| Molecular Weight(MW) | 82 kDa |
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NCAPH antibody can be used for detection of NCAPH by ELISA at 1:62500. NCAPH antibody can be used for detection of NCAPH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCAPH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
