Application
Western blot analysis of anti-NCAPH2 Pab in HepG2 cell line lysate (35ug/lane).| Product Name | NCAPH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Condensin-2 complex subunit H2, Chromosome-associated protein H2, hCAP-H2, Kleisin-beta, Non-SMC condensin II complex subunit H2, NCAPH2, CAPH2 |
| Product description | Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.1) Ono,T., et al. Cell 115 (1), 109-121 (2003). |
| Immunogen | This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NCAPH2 |
|---|---|
| Alternative Names | Condensin-2 complex subunit H2 Chromosome-associated protein H2 hCAP-H2 Kleisin-beta Non-SMC condensin II complex subunit H2 NCAPH2 CAPH2 |
| Molecular Weight(MW) | 68 kDa |
Application
Western blot analysis of anti-NCAPH2 Pab in HepG2 cell line lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
