Application
Immunocytochemistry of NCBP1 in HeLa cells with NCBP1 antibody at 2.5 μg/ml.Application
Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NCBP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCBP1 Antibody: NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein |
| Product description | NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).1) Ohno M, Kataoka N, and Shimura Y. A nuclear cap binding protein from HeLa cells. Nuc. Acid Res. 1990; 18:6989-95. |
| Immunogen | NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1. The immunogen is located within amino acids 420 - 470 of NCBP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCBP1 |
|---|---|
| Alternative Names | NCBP1 Antibody: NCBP Sto1 CBP80 NCBP Nuclear cap-binding protein subunit 1 80 kDa nuclear cap-binding protein |
| Molecular Weight(MW) | Predicted: 87 kDa Observed: 87 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1. |
Application
Immunocytochemistry of NCBP1 in HeLa cells with NCBP1 antibody at 2.5 μg/ml.
Application
Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NCBP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
