Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | NCKAP1L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCKAP1L, HEM1 |
| Product description | NCKAP1L is a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells, while hematopoietic protein 2 is preferentially expressed in brain, heart, liver and testis. The function of the HEM1 product has not been established but it is thought to play an essential role in oogenesis.The protein encoded by this gene is a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells, while hematopoietic protein 2 is preferentially expressed in brain, heart, liver and testis. The function of the HEM1 product has not been established but it is thought to play an essential role in oogenesis.1) Weiner, O.D., PLoS Biol. 5 (9), E221 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCKAP1L. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NCKAP1L antibody can be used for detection of NCKAP1L by ELISA at 1:62500. NCKAP1L antibody can be used for detection of NCKAP1L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCKAP1L |
|---|---|
| Alternative Names | NCKAP1L HEM1 |
| Molecular Weight(MW) | 128 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | NCKAP1L antibody can be used for detection of NCKAP1L by ELISA at 1:62500. NCKAP1L antibody can be used for detection of NCKAP1L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCKAP1L antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
