Application
Antibody used in IHC on Human NT-2 at 1:500.Application
Antibody used in WB on Human Daudi at 1.25 ug/ml.Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human NT-2 s at 2 ug/ml.| Product Name | NCL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCL, C23 |
| Product description | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.1) Huang, Y., (2006) Blood 107 (9), 3564-3571. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| NCL antibody can be used for detection of NCL by ELISA at 1:312500. NCL antibody can be used for detection of NCL by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NCL |
|---|---|
| Alternative Names | NCL C23 |
| Molecular Weight(MW) | 78 kDa |
Application
Antibody used in IHC on Human NT-2 at 1:500.
Application
Antibody used in WB on Human Daudi at 1.25 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human NT-2 s at 2 ug/ml.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | NCL antibody can be used for detection of NCL by ELISA at 1:312500. NCL antibody can be used for detection of NCL by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
