Application
Fluorescent confocal image of Hela cell stained wit. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). NucApplication
NCL Antibody (Center E443) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use o for immunohistochemistry.Application
Western blot analysis of NCL using rabbit polyclona using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | NCL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.1) Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010) |
| Immunogen | This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-455 amino acids from the Central region of human NCL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCL |
|---|---|
| Alternative Names | Nucleolin Protein C23 NCL |
| Molecular Weight(MW) | 77 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Fluorescent confocal image of Hela cell stained wit. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuc
Application
NCL Antibody (Center E443) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use o for immunohistochemistry.
Application
Western blot analysis of NCL using rabbit polyclona using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
