Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: U-2 OS whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NDC80 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2 |
| Product description | Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.1) Chen Y.,et al.Mol. Cell. Biol. 17:6049-6056(1997). |
| Immunogen | This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-539 amino acids from human NDC80. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDC80 |
|---|---|
| Alternative Names | Kinetochore protein NDC80 homolog Highly expressed in cancer protein Kinetochore protein Hec1 HsHec1 Kinetochore-associated protein 2 Retinoblastoma-associated protein HEC NDC80 HEC HEC1 KNTC2 |
| Molecular Weight(MW) | 74 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: U-2 OS whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
