Application
Immunohistochemistry staining of Nestin in kidney tissue using Nestin Antibody.| Product Name | Nestin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NES, Nestin, Nbla00170 |
| Product description | Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments. |
| Immunogen | Nestin antibody was raised against amino acids 1484 - 1500 of Nestin (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Nestin antibody can be used in ELISA starting at 1:1000 - 1:6000, Western Blot, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NES |
|---|---|
| Alternative Names | NES Nestin Nbla00170 |
Application
Immunohistochemistry staining of Nestin in kidney tissue using Nestin Antibody.| Application Notes | Nestin antibody can be used in ELISA starting at 1:1000 - 1:6000, Western Blot, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store Nestin antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
Data sheet for OM285991