Application
NETO2 Antibody (N-term ) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NETO2 Antibody (N-term ) for immunohistochemistry.Application
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit| Product Name | NETO2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, NETO2, BTCL2 |
| Product description | This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. It also has an intracellular FXNPXY-like motif, which has been shown in other proteins to be essential for the internalization of clathrin coated pits during endocytosis. Alternatively spliced transcript variants have been observed, but they have not been fully characterized. [provided by RefSeq].1) Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004) |
| Immunogen | This NETO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of human NETO2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NETO2 |
|---|---|
| Alternative Names | Neuropilin and tolloid-like protein 2 Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2 NETO2 BTCL2 |
| Molecular Weight(MW) | 59 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
NETO2 Antibody (N-term ) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NETO2 Antibody (N-term ) for immunohistochemistry.
Application
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
