Application
Western blot analysis of extracts from mouse brain tissue using NF- kappaB p65 Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | NF- kappaB p65 (phospho Ser281) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, p65 |
| Product description | NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.1) Hochrainer K, Racchumi G, Anrather J (2013)J Biol Chem 288, 285-93. Dai Y, et al. (2008) Clin Cancer Res 14, 549-58. Seldon MP, et al. (2007)J Immunol 179, 7840-51. |
| Immunogen | NF- kappaB p65 (phospho Ser281) antibody was raised against a peptide sequence around phosphorylation site of serine281 (E-L-S (p)-E-P) derived from Human NF- kappaB . |
| Modification | p-Ser281 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RELA |
|---|---|
| Alternative Names | NFKB3 Nuclear factor NF-kappa-B p65 subunit RELA TF65 p65 |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | The antibody detects endogenous level of NF- kappaB p65 only when phosphorylated at serine 281. |
| Entrez Gene | 5970 |
|---|---|
| Protein Accession | NP_001138610.1 |
Application
Western blot analysis of extracts from mouse brain tissue using NF- kappaB p65 Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
