Application
Western blot analysis of extracts from RAW264.7 cells treated with EGF using Phospho-NF-κB p100 (Ser872) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | NF-κB p100 (phospho Ser872) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Lyt10, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10 |
| Product description | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.1) Qu Z, Qing G, Rabson A, Xiao G (2004) J Biol Chem 279, 44563-72 . Xiao G, Fong A, Sun SC (2004) J Biol Chem 279, 30099-105 . |
| Immunogen | NF-κB p100 (phospho Ser872) antibody was raised against a peptide sequence around phosphorylation site of serine872 (S-Q-S (p)-V-E) derived from Human NF-κB p100 . |
| Modification | p-Ser872 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKB2 |
|---|---|
| Alternative Names | DNA-binding factor KBF2 Lymphocyte translocation chromosome 10 Lyt10 Nuclear factor NF-kappa-B p100 subunit Oncogene Lyt-10 |
| Molecular Weight(MW) | 100 kDa |
| Tissue Specificity | The antibody detects endogenous level of NF-κB p100 only when phosphorylated at serine 872. |
| Entrez Gene | 4791 |
|---|---|
| Protein Accession | NP_001070962.1 |
Application
Western blot analysis of extracts from RAW264.7 cells treated with EGF using Phospho-NF-κB p100 (Ser872) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
