NFIB is a member of the nuclear factor I family of nuclear proteins, which are known to be involved in viral and cellular transcription. NFIB includes the proposed DNA binding and dimerization domain. NFIB recognizes and binds the palindromic sequence 5'-1) Kimura, K., et al., (2006) Genome Res. 16 (1), 55-65.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIB.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NFIB antibody can be used for detection of NFIB by ELISA at 1:312500. NFIB antibody can be used for detection of NFIB by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
NFIB antibody can be used for detection of NFIB by ELISA at 1:312500. NFIB antibody can be used for detection of NFIB by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFIB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt