Application
Fluorescent confocal image of Hela cell stained with NFIC Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloidinApplication
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | NFIC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear factor 1 C-type, NF1-C, Nuclear factor 1/C, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/C, NF-I/C, NFI-C, TGGCA-binding protein, NFIC, NFI |
| Product description | NFIC recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This NFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-208 amino acids from the Central region of human NFIC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFIC |
|---|---|
| Alternative Names | Nuclear factor 1 C-type NF1-C Nuclear factor 1/C CCAAT-box-binding transcription factor CTF Nuclear factor I/C NF-I/C NFI-C TGGCA-binding protein NFIC NFI |
| Molecular Weight(MW) | 56 kDa |
Application
Fluorescent confocal image of Hela cell stained with NFIC Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
