Application
Western blot analysis of lysed extracts from HT29 cells using NFκB-p100/p52 (Ab-872).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFκB-p100/p52 (Ab-872). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using NFκB-p100/p52 (Ab-872).| Product Name | NFkB p100 / p52 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p52, p105, H2TF1, LYT10, CVID10, LYT-10, NF-kB2, FLK1, VEGFR2, DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Lyt10, NFKB2 |
| Product description | NF-κ-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.1) Shin HM, et al. (2006) EMBO J; 25 (1): 129-138. Li Q, et al. (2005) Proc Natl Acad Sci USA; 102 (35): 12425-12430. Chen C, et al. (2000) Mol Cell Biol; 20 (8): 2687-2695. |
| Immunogen | NFkB-p100/p52 (Ab-872) antibody was raised against a peptide sequence around aa.870~874 (S-Q-S-V-E) derived from Human NFκB-p100/p52. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKB2 |
|---|---|
| Alternative Names | p52 p105 H2TF1 LYT10 CVID10 LYT-10 NF-kB2 FLK1 VEGFR2 DNA-binding factor KBF2 Lymphocyte translocation chromosome 10 Lyt10 NFKB2 |
| Molecular Weight(MW) | 52, 120 kDa |
| Tissue Specificity | This antibody detects endogenous level of total NFκB-p100/p52 protein. |
| Entrez Gene | 4791 |
|---|---|
| Protein Accession | NP_001070961.1 |
Application
Western blot analysis of lysed extracts from HT29 cells using NFκB-p100/p52 (Ab-872).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFκB-p100/p52 (Ab-872). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using NFκB-p100/p52 (Ab-872).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
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| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
