Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p105/p50 (Phospho-Ser927). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using NFκB-p105/p50 (Phospho-Ser927).Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with TNF-α using NFκB-p105/p50 (Phospho-Ser927).| Product Name | NFkB p105 / p50 (phospho Ser927) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B, SHC, SHCA, DNA-binding factor KBF1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50 |
| Product description | NF-κ-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.1) Hou S, et al. (2003) J Biol Chem. 278 (46): 45994-45998. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289. |
| Immunogen | NFkB-p105/p50 (Phospho-Ser927) antibody was raised against a peptide sequence around phosphorylation site of serine 927 (C-D-S (p) -G-V) derived from Human NFκB-p105. |
| Modification | p-Ser927 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKB1 |
|---|---|
| Alternative Names | p50 KBF1 p105 EBP-1 NF-kB1 NFKB-p50 NFkappaB NF-kappaB NFKB-p105 NF-kappa-B SHC SHCA DNA-binding factor KBF1 NF-kappa-B1 p84/NF-kappa-B1 p98 NFKB1 NFkB-p50 |
| Molecular Weight(MW) | 50, 120 kDa |
| Tissue Specificity | This antibody detects endogenous level of NFκB-p105/p50 only when phosphorylated at serine 927. |
| Entrez Gene | 4790 |
|---|---|
| Protein Accession | NP_001158884.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p105/p50 (Phospho-Ser927). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using NFκB-p105/p50 (Phospho-Ser927).
Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with TNF-α using NFκB-p105/p50 (Phospho-Ser927).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
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| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
