Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Thr254). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa using NFκB-p65(Phospho-Thr254).Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Thr254).| Product Name | NFkB p65 (phospho Thr254) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p65, NFKB3, CTNNB, OK/SW-cl.35, PRO2286, RELA, TF65, Transcription factor p65, p65 |
| Product description | NF-κ-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κ-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-κ-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κ-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κ-B complex which translocates to the nucleus. NF-κ-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-κ-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-κ-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-κ-B complex.1) Yeh PY, et al. (2004) J Biol Chem. 279 (25): 26143-26148. Ryo A, et al. (2003) Mol Cell. 12 (6): 1413-1426. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289. |
| Immunogen | NFkB-p65 (Phospho-Thr254) antibody was raised against a peptide sequence around phosphorylation site of threonine 254 (F-R-T (p) -P-P) derived from Human NFκB-p65. |
| Modification | p-Thr254 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RELA |
|---|---|
| Alternative Names | p65 NFKB3 CTNNB OK/SW-cl.35 PRO2286 RELA TF65 Transcription factor p65 p65 |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | This antibody detects endogenous level of NFκB p65 only when phosphorylated at threonine 254. |
| Entrez Gene | 5970 |
|---|---|
| Protein Accession | NP_001138610.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Thr254). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa using NFκB-p65(Phospho-Thr254).
Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Thr254).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
