Application
Western Blot at 1:1000 dilution Lane 1: Raji whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NFKB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p50 subunit, NFKB1 |
| Product description | NFKB1 is a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.1) Legembre, P., et al., J. Biol. Chem. 279(45):46742-46747 (2004). |
| Immunogen | This NFKB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-939 amino acids from human NFKB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKB1 |
|---|---|
| Alternative Names | Nuclear factor NF-kappa-B p105 subunit DNA-binding factor KBF1 EBP-1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 Nuclear factor NF-kappa-B p50 subunit NFKB1 |
| Molecular Weight(MW) | 105 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western Blot at 1:1000 dilution Lane 1: Raji whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
