NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth.1) Xiao, G., et al., (2004) J. Biol. Chem. 279 (29), 30099-30105.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKB2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NFKB2 antibody can be used for detection of NFKB2 by ELISA at 1:1562500. NFKB2 antibody can be used for detection of NFKB2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Lung.
Positive Control
1) Cat. No. 1207 - Raji Cell Lysate
Application Notes
NFKB2 antibody can be used for detection of NFKB2 by ELISA at 1:1562500. NFKB2 antibody can be used for detection of NFKB2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFKB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.