Application
Antibody used in IHC on Human Muscle.Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Liver cell lysates.| Product Name | NFYA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFYA, HAP2, CBF-A, CBF-B, NF-YA |
| Product description | NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.1) Zhu, Q.S., et al., (2004) J.Biol.Chem.279(29), 29902-29910. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| NFYA antibody can be used for detection of NFYA by ELISA at 1:62500. NFYA antibody can be used for detection of NFYA by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFYA |
|---|---|
| Alternative Names | NFYA HAP2 CBF-A CBF-B NF-YA |
| Molecular Weight(MW) | 37 kDa, 34 kDa |
Application
Antibody used in IHC on Human Muscle.
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver cell lysates.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NFYA antibody can be used for detection of NFYA by ELISA at 1:62500. NFYA antibody can be used for detection of NFYA by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFYA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
