Application
Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 5 μg/mL.Application
Western blot analysis of Nicastrin in mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 μg/mL.| Product Name | Nicastrin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nicastrin Antibody: ATAG1874, KIAA0253, UNQ1874/PRO4317, Nicastrin |
| Product description | Nicastrin Antibody: Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.1) Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8. |
| Immunogen | Nicastrin antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin. The immunogen is located within amino acids 630 - 680 of Nicastrin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Nicastrin antibody can be used for detection of Nicastrin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCSTN |
|---|---|
| Alternative Names | Nicastrin Antibody: ATAG1874 KIAA0253 UNQ1874/PRO4317 Nicastrin |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 5 μg/mL.
Application
Western blot analysis of Nicastrin in mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | Nicastrin antibody can be used for detection of Nicastrin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Nicastrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nicastrin Antibody is supplied in PBS containing 0.02% sodium azide. |
