Application
Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.Application
Immunofluorescence of NINJ1 in mouse brain tissue with NINJ1 antibody at 20 μg/ml.Application
Western blot analysis of NINJ1 in human brain tissue lysate with NINJ1 antibody at 1 μg/ml.| Product Name | NINJ1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NINJ1 Antibody: NIN1, NINJURIN, Ninjurin-1, Nerve injury-induced protein 1 |
| Product description | Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).1) Araki T and Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron 1996; 17:353-61. |
| Immunogen | NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1. The immunogen is located within the last 50 amino acids of NINJ1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NINJ1 |
|---|---|
| Alternative Names | NINJ1 Antibody: NIN1 NINJURIN Ninjurin-1 Nerve injury-induced protein 1 |
| Molecular Weight(MW) | Predicted: 17 kDa Observed: 16 kDa |
| Tissue Specificity | NINJ1 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.
Application
Immunofluorescence of NINJ1 in mouse brain tissue with NINJ1 antibody at 20 μg/ml.
Application
Western blot analysis of NINJ1 in human brain tissue lysate with NINJ1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NINJ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NINJ1 antibody is supplied in PBS containing 0.02% sodium azide. |
