Application
Western blot analysis of NIP7 using rabbit polyclonal NIP7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NIP7 gene.Application
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NIP7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 60S ribosome subunit biogenesis protein NIP7 homolog, KD93, NIP7 |
| Product description | Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.1) Liu,J.F., et.al., J. Struct. Biol. 148 (3), 370-374 (2004) |
| Immunogen | This NIP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-177 amino acids from the C-terminal region of human NIP7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NIP7 |
|---|---|
| Alternative Names | 60S ribosome subunit biogenesis protein NIP7 homolog KD93 NIP7 |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of NIP7 using rabbit polyclonal NIP7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NIP7 gene.
Application
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
