NIPSNAP2 Antibody: The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. It is widely expressed and most abundant in heart and skeletal muscle. NIPSNAP proteins have been suggested to be important in vesicular transport. NIPSNAP2 antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.1) Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene1998; 212:13-20.
Immunogen
NIPSNAP2 antibody was raised against a 14 amino acid synthetic peptide near the center of human NIPSNAP2. The immunogen is located within the first 50 amino acids of NIPSNAP2.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
NIPSNAP2 antibody can be used for detection of NIPSNAP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.:
Immunohistochemistry of NIPSNAP2 in mouse skeletal muscle tissue with NIPSNAP2 antibody at 2.5 μg/mL.
Application
Western blot analysis of NIPSNAP2 in human skeletal muscle tissue lysate with NIPSNAP2 antibody at (A) 1 and (B) 2 μg/mL.
Positive Control
1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Application Notes
NIPSNAP2 antibody can be used for detection of NIPSNAP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
NIPSNAP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
NIPSNAP2 Antibody is supplied in PBS containing 0.02% sodium azide.