Application
Immunofluorescence of NK3R in Human Brain tissue with NK3R antibody at 20 ug/mL.Application
Immunohistochemistry of NK3R in human brain tissue with NK3R antibody at 5 μg/mL.Application
Western blot analysis of NK3R in RAW264.7 cell lysate with NK3R antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | NK3R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NK3R Antibody: NKR, HH11, NK3R, NK-3R, TAC3RL, TAC3R, Neuromedin-K receptor, NK-3 receptor, NKR |
| Product description | NK3R Antibody: The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B.1) Maggi CA. The mammalian tachykinin receptors. Gen. Pharmacol. 1995; 26:911-44. |
| Immunogen | NK3R antibody was raised against a 18 amino acid synthetic peptide from near the center of human NK3R. The immunogen is located within amino acids 370 - 420 of NK3R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TACR3 |
|---|---|
| Alternative Names | NK3R Antibody: NKR HH11 NK3R NK-3R TAC3RL TAC3R Neuromedin-K receptor NK-3 receptor NKR |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 51 kDa |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | NK3R antibody does not recognize NK1R or NK2R. |
Application
Immunofluorescence of NK3R in Human Brain tissue with NK3R antibody at 20 ug/mL.
Application
Immunohistochemistry of NK3R in human brain tissue with NK3R antibody at 5 μg/mL.
Application
Western blot analysis of NK3R in RAW264.7 cell lysate with NK3R antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NK3R antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NK3R Antibody is supplied in PBS containing 0.02% sodium azide. |
