Application
Immunohistochemistry of NKX2-2 in human kidney tissue with NKX2-2 antibody at 2.5 μg/mL.Application
Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of NKX2-2 in human kidney tissue with NKX2-2 antibody at 20 μg/mL.| Product Name | NKX2-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B |
| Product description | NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.1) Sussel L, Kalamaras J, Hartigan-O’Connor DJ, et al. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 1998; 125:2213-21 |
| Immunogen | NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2. The immunogen is located within amino acids 90 - 140 of NKX2-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKX2-2 |
|---|---|
| Alternative Names | NKX2-2 Antibody: NKX2B NKX2.2 NKX2B Homeobox protein NK-2 homolog B |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members. |
Application
Immunohistochemistry of NKX2-2 in human kidney tissue with NKX2-2 antibody at 2.5 μg/mL.
Application
Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of NKX2-2 in human kidney tissue with NKX2-2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NKX2-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NKX2-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
