Nkx6.3 is a new member of the Nkx6 subfamily of homeodomain proteins. Members of the Nkx family of homeodomain proteins are involved in a variety of developmental processes such as cell fate determination in the CNS and in the pancreas. Nkx6.3 is expressed in the developing CNS and gastro-intestinal tract. Nkx6.3 shows a remarkably selective expression in a subpopulation of differentiating V2 neurons at caudal hindbrain levels. In the gut, Nkx6.3 is expressed in duodenal and glandular stomach endoderm and at the end of gestation Nkx6.3 became restricted to the base of the gastric units in the glandular stomach.1) Sugano, S. Unpublished (2001).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NKX6-3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NKX6-3 antibody can be used for detection of NKX6-3 by ELISA at 1:312500. NKX6-3 antibody can be used for detection of NKX6-3 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
NKX6-3 antibody can be used for detection of NKX6-3 by ELISA at 1:312500. NKX6-3 antibody can be used for detection of NKX6-3 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NKX6-3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt