Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Confocal immunofluorescent analysis of NM23 (NME1) Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | NM23 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23 |
| Product description | NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. NME1 encodes the 'A' isoform of nucleoside diphosphate kinase (NDK). NDK exists as a hexamer composed of the 'A' (NME1) and 'B' (encoded by NME2) isoforms. Mutations in NME1 have been identified in aggressive neuroblastomas.1) Munier, A., et al., Exp. Cell Res. 289(2):295-306 (2003). |
| Immunogen | This NM23 (NME1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NM23 (NME1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NME1 |
|---|---|
| Alternative Names | Nucleoside diphosphate kinase A NDK A NDP kinase A Granzyme A-activated DNase GAAD Metastasis inhibition factor nm23 NM23-H1 Tumor metastatic process-associated protein NME1 NDPKA NM23 |
| Molecular Weight(MW) | 17 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Confocal immunofluorescent analysis of NM23 (NME1) Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
