Application
Confocal immunofluorescent analysis of NMB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
NMB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | NMB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuromedin-B, Neuromedin-B-32, Neuromedin-B, NMB |
| Product description | NMB stimulates smooth muscle contraction in a manner similar to that of bombesin.1) Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008) |
| Immunogen | This NMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-42 amino acids from the Central region of human NMB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NMB |
|---|---|
| Alternative Names | Neuromedin-B Neuromedin-B-32 Neuromedin-B NMB |
| Molecular Weight(MW) | 13 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of NMB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
NMB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
