NOBOX is a transcription factor which may play a role in oogenesis. It binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'.1) Huntriss J, (2006) Mol Hum Reprod. 2006 May;12(5):283-9..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOBOX.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NOBOX antibody can be used for detection of NOBOX by ELISA at 1:312500. NOBOX antibody can be used for detection of NOBOX by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in IHC on Human Heart.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
NOBOX antibody can be used for detection of NOBOX by ELISA at 1:312500. NOBOX antibody can be used for detection of NOBOX by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOBOX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.