Application
Immunocytochemistry of NOD4 in HeLa cells with NOD4 antibody at 10 μg/mL.Application
Western blot analysis of NOD4 in EL4 cell lysate with NOD4 antibody at 1 μg/mL.Application
Immunofluorescence of NOD4 in HeLa cells with NOD4 antibody at 20 μg/mL.| Product Name | NOD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOD4 Antibody: NOD4, NOD27, CLR16.1, NOD4, Protein NLRC5, Nucleotide-binding oligomerization domain protein 27 |
| Product description | NOD4 Antibody: NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-κB and type I interferon signaling, NOD4 may be a useful target for manipulating immune responses against infectious or inflammation-associated diseases, including cancer.1) Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci. 2006; 1072:19-27. |
| Immunogen | NOD4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NOD4. The immunogen is located within amino acids 20 - 70 of NOD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| NOD4 antibody can be used for detection of NOD4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRC5 |
|---|---|
| Alternative Names | NOD4 Antibody: NOD4 NOD27 CLR16.1 NOD4 Protein NLRC5 Nucleotide-binding oligomerization domain protein 27 |
| Molecular Weight(MW) | 205 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins. |
Application
Immunocytochemistry of NOD4 in HeLa cells with NOD4 antibody at 10 μg/mL.
Application
Western blot analysis of NOD4 in EL4 cell lysate with NOD4 antibody at 1 μg/mL.
Application
Immunofluorescence of NOD4 in HeLa cells with NOD4 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | NOD4 antibody can be used for detection of NOD4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NOD4 Antibody is supplied in PBS containing 0.02% sodium azide. |
