Application
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 μg/mL.Application
Immunofluorescence of NogoA in Mouse Brain cells with NogoA antibody at 20 μg/mL.Application
Western blot analysis of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | NogoA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO-A, NSP-CL, Nogo-B, Nogo-C, RTN4-A, RTN4-C, RTN4-B1, RTN4-B2, NI220/250, Nbla00271, Nbla10545, KIAA0886, My043, SP1507, Reticulon-4, Foocen, Nogo protein |
| Product description | NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.1) Yan R, ShiQ, Hu X, et al. Reticulon proteins: emerging players in neurodegenerative diseases. Cell. Mol. Life Sci.2006; 63:877-889. |
| Immunogen | NogoA antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA. The immunogen is located within the first 50 amino acids of NogoA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RTN4 |
|---|---|
| Alternative Names | NogoA Antibody: ASY NSP NOGO NOGOC RTN-X NOGO-A NSP-CL Nogo-B Nogo-C RTN4-A RTN4-C RTN4-B1 RTN4-B2 NI220/250 Nbla00271 Nbla10545 KIAA0886 My043 SP1507 Reticulon-4 Foocen Nogo protein |
| Tissue Specificity | At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA. |
Application
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 μg/mL.
Application
Immunofluorescence of NogoA in Mouse Brain cells with NogoA antibody at 20 μg/mL.
Application
Western blot analysis of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NogoA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NogoA Antibody is supplied in PBS containing 0.02% sodium azide. |
