Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Product Name | NOL6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOL6, FLJ21959, MGC14896, MGC14921, MGC20838, NRAP, UTP22, bA311H10.1 |
| Product description | NOL6 is a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified.1) Nousiainen, M., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (14), 5391-5396. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOL6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NOL6 antibody can be used for detection of NOL6 by ELISA at 1:1562500. NOL6 antibody can be used for detection of NOL6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOL6 |
|---|---|
| Alternative Names | NOL6 FLJ21959 MGC14896 MGC14921 MGC20838 NRAP UTP22 bA311H10.1 |
| Molecular Weight(MW) | 127 kDa |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | NOL6 antibody can be used for detection of NOL6 by ELISA at 1:1562500. NOL6 antibody can be used for detection of NOL6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOL6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
