Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.WB
Notch1 and Hes-1 protein expression were analyzed by Western blot 48 h after miRNA transfection by UTMD.| Product Name | NOTCH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NOTCH1 is a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Sahlgren, C., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (17), 6392-6397. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOTCH1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NOTCH1 antibody can be used for detection of NOTCH1 by ELISA at 1:312500. NOTCH1 antibody can be used for detection of NOTCH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOTCH1 |
|---|---|
| Alternative Names | NOTCH1 TAN1 hN1 |
| Molecular Weight(MW) | 88 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
WB
Notch1 and Hes-1 protein expression were analyzed by Western blot 48 h after miRNA transfection by UTMD.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | NOTCH1 antibody can be used for detection of NOTCH1 by ELISA at 1:312500. NOTCH1 antibody can be used for detection of NOTCH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOTCH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
