Application
Immunohistochemistry staining of NOTCH1 in cerebellum tissue using NOTCH1 Antibody.| Product Name | NOTCH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOTCH1, Notch 1, TAN1 |
| Product description | Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. |
| Immunogen | NOTCH1 antibody was raised against amino acids 2488-2502 from the C-Terminus of NOTCH1 (Human). A residue of cysteine was added to the amino terminal end to facilitate coupling. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| NOTCH1 antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOTCH1 |
|---|---|
| Alternative Names | NOTCH1 Notch 1 TAN1 |
Application
Immunohistochemistry staining of NOTCH1 in cerebellum tissue using NOTCH1 Antibody.| Application Notes | NOTCH1 antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NOTCH1 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
