Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on Human Breast at 5.0 ug/ml.| Product Name | NOTCH4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOTCH4, INT3, NOTCH3 |
| Product description | NOTCH4 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. NOTCH4 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. NOTCH4 functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. NOTCH4 gene may be associated with susceptibility to schizophrenia in a small portion of cases.1) Sugaya K., et al., (1997) Gene 189:235-244. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOTCH4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NOTCH4 antibody can be used for detection of NOTCH4 by ELISA at 1:312500. NOTCH4 antibody can be used for detection of NOTCH4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOTCH4 |
|---|---|
| Alternative Names | NOTCH4 INT3 NOTCH3 |
| Molecular Weight(MW) | 65 kDa, 210 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Breast at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NOTCH4 antibody can be used for detection of NOTCH4 by ELISA at 1:312500. NOTCH4 antibody can be used for detection of NOTCH4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOTCH4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
