Application
Immunofluorescence of NOX4 in human spleen tissue with NOX4 antibody at 20 μg/ml.Application
Immunohistochemistry of NOX4 in human spleen tissue with NOX4 antibody at 5 μg/ml.Application
Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | NOX4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAPDH oxidase 4, NOX-4, RENOX, KOX, KOX-1 |
| Product description | The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.1) Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313. |
| Immunogen | NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4. The immunogen is located within amino acids 70 - 120 of NOX4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOX4 |
|---|---|
| Alternative Names | NAPDH oxidase 4 NOX-4 RENOX KOX KOX-1 |
| Molecular Weight(MW) | Predicted: 64 kDa Observed: 68 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins. |
Application
Immunofluorescence of NOX4 in human spleen tissue with NOX4 antibody at 20 μg/ml.
Application
Immunohistochemistry of NOX4 in human spleen tissue with NOX4 antibody at 5 μg/ml.
Application
Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NOX4 antibody is supplied in PBS containing 0.02% sodium azide. |
