Application
Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 antibody at 2.5 μg/mL.Application
Western blot analysis of NPC1 in HepG2 cell lysate with NPC1 antibody at 1 μg/mL.Application
Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 μg/mL.| Product Name | NPC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPC1 Antibody: NPC, Niemann-Pick C1 protein |
| Product description | NPC1 Antibody: Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations. This anti-NPC1 antibody will not cross-react to NPC2, another gene whose defects also result in Niemann-Pick type C disease.1) Karten B, Peake KB, and Vance JE. Mechanisms and consequences of impaired lipid trafficking in Niemann-Pick type C1-deficient mammalian cells. Biochim. Biophys. Acta2009; 1791:656-70. |
| Immunogen | NPC1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human NPC1. The immunogen is located within the last 50 amino acids of NPC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NPC1 antibody can be used for detection of NPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPC1 |
|---|---|
| Alternative Names | NPC1 Antibody: NPC Niemann-Pick C1 protein |
| Molecular Weight(MW) | Predicted: 140 kDa Observed: 150 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (87%) |
Application
Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 antibody at 2.5 μg/mL.
Application
Western blot analysis of NPC1 in HepG2 cell lysate with NPC1 antibody at 1 μg/mL.
Application
Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NPC1 antibody can be used for detection of NPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NPC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NPC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
